Microsatellite Marker Content Mapping of 12 Candidate Genes for Obesity: Assembly of Seven Obesity Screening Panels for Automated Genotyping

Table 1.

Candidate Gene Screening Panels

Candidate gene STS Size range Chromosomal location Type of repeat Dye
Candidate Panel 1
Leptin D7S530 106–118 7q31–35 2 FAM
CPE D4S417 174–186 4pter–qter 2 FAM
β-3AR D8S1803 266–288 8pter–qter 2 FAM
Leptin D7S514 147–157 7 2 TET
NPY Y5R D4S1603 190–208 4pter–qter 2 TET
POMC D2S171 253–281 2p24–p21 2 TET
UCP2 D11S916 135–153 11q13–q23 2 HEX
β-3AR D8S1791 225–243 8pter–qter 2 HEX
ART D20S106 316–322 20 2 HEX
Candidate Panel 2
NPY Y5R D4S3046 93–103 4pter–qter 2 FAM
tub D11S932 150–164 11p15.4–15.1 2 FAM
PPARγ D3S3610 249–263 3 2 FAM
Ob-R D1S198 308–322 1p32 2 FAM
tub D11S909 113–125 11p15.4 2 TET
PPARγ D3S3701 171–179 3 2 TET
ART D16S496 209–226 16q22.1 2 TET
Ob-R D1S2866 277–287 1p32 2 TET
PPARγ D3S3602 114–132 3 2 HEX
CPE D4S1566 197–209 4pter–qter 2 HEX
UCP2 D11S4207 256–282 11pter–qter 2 HEX
Candidate Panel 3
NPY D7S673 118–148 7p22–p21 2 FAM
MC4R D18S64 188–208 18q21.32 2 FAM
NPY D7S682 271–283 7pter–qter 2 FAM
NPY Y6R D5S1983 112–122 5 2 TET
NPY Y6R D5S414 186–206 5 2 TET
ART D16S3019 240–254 16pter–qter 2 TET
PC 1 D5S644 81–101 5 2 HEX
APOJ D8S137 152–162 8p21.3–q11.1 2 HEX
ART D16S400 192–202 16q21 2 HEX
PC 1 D5S484 261–281 5 2 HEX
Candidate Panel 4
POMC D2S2170 130–134 2qter 2 FAM
POMC D2S144 152–200 2p24–p21 2 FAM
leptin D7S635 216–234 7 2 FAM
ART D16S3021 156–174 16pter–qter 2 TET
POMC D2S2168 199–229 2qter 2 TET
Ob-R D1S2852 246–278 1p31 2 TET
APOJ D8S1809 154–174 8 2 HEX
tub D11S1331 191–205 11p15.4–15.1 2 HEX
β-3AR D8S1791 225–243 8qter–pter 2 HEX
Candidate Panel 5
MC4R D18S1357 126–147 18q21.32 4 FAM
NPY Y6R D5S816 225–253 5 4 FAM
NPY Y5R/CPE D4S3339 267–283 4pter–qter 4 FAM
NPY D7S1821 308–324 7pter–qter 4 FAM
NPY D7S2190 134–149 7pter–qter 3 TET
PC 1 GATA3H06 195–241 5 4 TET
β-3AR D8S2317 283–295 8 4 TET
UCP2 D11S2371 193–213 11pter–qter 4 HEX
MC4R GATA89B12 260 18q21.32 4 HEX
Obesity syndrome STS Size range Chromosomal location Type of repeat Dye
Syndrome Panel 1
CS D8S257 106–122 8q22.2 2 FAM
BBS2 D16S419 146–164 16q12.1 2 FAM
PW D15S128 193–209 15q11–13 2 FAM
BBS4 D15S204 116–134 15q22.3–23 2 TET
SS D12S79 150–180 12 2 TET
PW-paternal D15S156 217–229 15q11–13 2 TET
BBS3 D13S1251 125–139 3p11–q11 2 HEX
BBS4 D15S125 157–169 15q22.3–23 2 HEX
CS D8S559 218–230 8q22.2 2 HEX
Syndrome Panel 2
BBS1 D11S987 82–118 11q13.1 2 FAM
BBS3 D3S1271 146–158 3p11–q11 2 FAM
BBS1 D11S480 189–201 11q13.1 2 FAM
BBS1 D11S913 220 11q13 2 FAM
PW D15S210 130–146 15q11–13 2 TET
BBS4 D15S114 177–187 15q22.3–23 2 TET
BBS4 D15S131 238–274 15q22.3–23 2 TET
AS D15S122 143–159 15q11.2–13 2 HEX
BBS2 D16S408 241–251 16q13 2 HEX

  • Candidate screening panels 1–4 contain dinucleotide repeat polymorphic microsatellite markers for obesity candidate genes. There are six separate subheadings within each panel. Under Candidate gene, the gene for which the STS is a marker is identified. Under STS, the Whitehead designated name of the microsatellite is listed. Under size range, the established CEPH expected size range for the STS is listed. Under chromosomal location, the most precise chromosomal address as indicated in the Genome Database is listed. All polymorphic markers included in panels 1–4 are dinucleotide repeats. Under Dye, the recommended fluorescent dye used to label the STS and maximize the power of the technology is listed. Obesity candidate screening panel 5 and Syndrome panels 1 and 2 are named. Candidate panel 5 contains all tetranucleotide repeat STS, with the exception of D7S2190, which is a trinucleotide repeat microsatellite. Under Obesity syndrome, (panels 1 and 2) the obesity-related syndrome is listed for which the individual markers are used to assay.

This Article

  1. Genome Res. 8: 985-994

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