A High-Resolution Microsatellite Map of the Mouse Genome

Table 3.

Number and Distribution of Recombinants by Chromosome

Recomb. class Chromosome
1 2 3 4 5 6 7 8 9 10 11
 0 316 332 447 350 312 376 443 395 433 448 438
(282) (281) (418) (318) (291) (342) (371) (347) (414) (400) (490)
 1 378 352 369 382 407 395 344 385 401 366 437
(352) (352) (367) (359) (354) (361) (361) (361) (358) (359) (341)
 2 141 157 80 119 130 89 69 84 60 69 88
(220) (220) (152) (202) (215) (190) (176) (188) (154) (161) (118)
 3 81 67 57 71 72 71 76 66 50 56 17
(91) (92) (43) (76) (87) (67) (57) (65) (44) (48) (27)
 4 33 42 19 40 28 27 22 15 22 19 0
(29) (29) (9) (21) (27) (18) (14) (17) (10) (11) (5)
 5 19 16 4 10 16 14 19 24 10 17 1
(7) (7) (2) (5) (7) (4) (3) (4) (2) (2) (1)
 6 8 11 3 7 10 5 5 6 3 2 0
(2) (2) (1) (1) (1) (1)
 7 4 1 1 2 2 2 2 3 3 4 0
 8 1 2 1 1 2 2 1 2 0 1 0
 9 0 0 1 0 1 0 0 1 0 0 0
10 0 1 0 0 2 0 1 1 0 0 0
11 1 0 0 0 0 1 0 0 0 0 0
12 0 0 0 0 0 0 0 0 0 0 0
13 0 0 0 0 0 0 0 0 0 0 1
14 0 0 0 0 0 0 0 0 0 0 0
15 0 1 0 0 0 0 0 0 0 0 0
χ2 101.38 98.9 70.03 89.07 123.72 150.85 282.99 317.6 125.38 128.19 46.81
Total  recomb. 1225 1229 838 1107 1194 1035 955 1023 848 881 682
Length (cM) 90 95 77 84 92 78 71 62 73 68 72
Binomial test 16.151* 4.78 11.269* 7.302 5.14 8.032 9.9* 99.353* 2.015 3.524 45.725*
Recomb. class Chromosome Total chr. Total recombs. Percent Total Chr.
12 13 14 15 16 17 18 19 X
 0 462 475 578 503 507 474 467 483 410 8649 0 44.038
(475) (450) (547) (552) (557) (530) (457) (505) (425)
 1 406 383 329 429 417 445 397 412 429 7863 7863 40.036
(345) (351) (320) (318) (316) (327) (350) (336) (356)
 2 50 42 12 21 38 31 18 36 72 1406 2812 7.159
(125) (137) (94) (92) (90) (101) (134) (112) (149)
 3 56 58 48 24 18 29 93 42 47 1099 3297 5.596
(30) (36) (18) (18) (17) (21) (34) (25) (42)
 4 4 8 2 4 0 3 0 3 17 308 1232 1.568
(6) (7) (3) (3) (2) (3) (7) (4) (9)
 5 2 10 9 0 2 0 5 4 4 186 930 0.947
(1) (1) (1) (1) (2)
 6 1 3 3 0 0 0 1 2 1 71 426 0.362
 7 1 1 1 1 0 0 0 0 2 30 210 0.153
 8 0 1 0 0 0 0 0 0 0 14 112 0.071
 9 0 0 0 0 0 0 1 0 0 4 36 0.020
10 0 0 0 0 0 0 0 0 0 5 50 0.025
11 0 1 0 0 0 0 0 0 0 3 33 0.015
12 0 0 0 0 0 0 0 0 0 0 0 0.000
13 0 0 0 0 0 0 0 0 0 1 13 0.076
14 0 0 0 0 0 0 0 0 0 0 0 0.000
15 0 0 0 0 0 0 0 0 0 1 15 0.088
χ2 78.53 114.52 168.93 101.41 66.55 100.31 208.27 85.23 73.88
Total  recomb. 713 767 575 566 557 606 752 654 822 17029
Length (cM) 60 68 53 62 56 59 55 54 69
Binomial test 0.456 4.77 8.023 49.609* 23.913* 18.445* 10.46* 0.022 0.428
  • The number of nonrecombinant and recombinant classes observed for each chromosome are listed along with the numbers of classes expected according to a Poisson distribution (in parentheses). Where the expected number is <1, no figure is given. χ2 values from goodness-of-fit tests applied to each chromosome are given. All chromosomes differ very significantly from a Poisson distribution at p < 10−9. Although there appears to be a broad relationship between numbers of total recombinants per chromosome and genetic length (cM), the observed distribution deviates significantly from that expected if the number of recombinants varies in proportion to genetic length (χ2 = 313.64, P < 10−10; 19df.). A binomial test of significance has also been applied to each chromosome to identify those chromosomes that depart most significantly from expected, and the χ2values for each chromosome are listed. We have taken account of the likelihood of false positives, i.e., chromosomes that depart from expected by chance. Monte Carlo simulations indicate that when adopting a significance level of 0.05, one false-positive chromosome will arise with a frequency of 0.6317. Reducing the false-positive rate to 0.05 the binomial test needs to be applied at a significance level of 0.002. At α = 0.002 chromosomes 1, 3, 7, 8, 11, 15, 16, 17 and 18 show a significant departure from expected (indicated by asterisk).

This Article

  1. Genome Res. 8: 531-542

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