Method

Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing

    • Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
Published March 30, 2012. Vol 22 Issue 6, pp. 1139-1143. https://doi.org/10.1101/gr.136242.111
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cover of Genome Research Vol 36 Issue 4
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April 2026, Vol. 36, No. 4
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Abstract

We have adapted transposase-based in vitro shotgun library construction (“tagmentation”) for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-quality coverage of each strand.

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