Molecular subtypes of B. pseudomallei.(A) Hierarchical clustering of all Burkholderia strains. B. pseudomallei strains can be grouped into three distinct subtypes (G1, G2, G3), further confirmed by the plot of correlation between each strain and the `average' or `representative' profiles of B. mallei, B. thailandensis, and subtypes G1, G2, and G3 on the right. Numbers at the left of each node represent the number of ORFs that differentiate between the two branches of the node at a confidence level of 99.99% and above. (B) Principal components analysis (PCA) of B. pseudomallei strains based on the top 6000 most highly varying genetic elements. Axes P1, P2, and P3 refer to first, second, and third principal components, respectively. Groupings similar to those in (A) are observed. (C) PCA of B. pseudomallei strains based on P3 and P4. Circled `X's represent centroids of each subtype G1, G2, and G3. A circle is drawn from each centroid to the most deviant member of the corresponding group, showing that none of these circles overlap each other. Distributions similar to those in (A) are observed.
