ARTICLE

Simplified template preparation and improved direct sequencing using Taq polymerase.

    • Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Published May 1, 1992. Vol 1 Issue 4, pp. 255-256. https://doi.org/10.1101/gr.1.4.255
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cover of Genome Research Vol 36 Issue 6
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June 2026, Vol. 36, No. 6
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Abstract

A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.

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