ARTICLE

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

    • Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.
Published February 1, 1992. Vol 1 Issue 3, pp. 160-163. https://doi.org/10.1101/gr.1.3.160
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cover of Genome Research Vol 36 Issue 4
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April 2026, Vol. 36, No. 4
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Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

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