A Gene Map of the Best’s Vitelliform Macular Dystrophy Region in Chromosome 11q12–q13.1
Table 1 Fine Mapping and Characterization of ESTs
|
Mapping data of the assigned UniGene EST clusterb |
Refined mapping of ESTs relative to the Best's disease locus |
|||||||||||
|
EST designationa |
Acc. No. |
RH position (cR) |
Distance from pter (cM) |
Interval D11S loci |
Method |
PAC/YAC Id |
Localization to D11S1765-UGB |
Northern Blot analysis |
Transcription unit |
|||
|
EST236824 |
R60069 |
303.65 |
62-65 |
S1357-S1765 |
PCR |
942_g_8 |
no, prox. to D11S1765 |
n.v. |
- |
|||
|
EST235648 |
R58859 |
305.95 |
62-65 |
S1357-S1765 |
PCR |
942_g_8 |
no, prox. to D11S1765 |
n.v. |
- |
|||
|
EST385176 |
H82877 |
305.95 |
62-65 |
S1357-S1765 |
PCR |
942_g_8 |
no, prox. to D11S1765 |
n.v. |
- |
|||
|
EST176668 |
R06372 |
317.16 |
62-70 |
S1357-S913 |
PCR |
942_g_8 |
no, prox. to D11S1765 |
n.v. |
- |
|||
|
EST384727 |
R99306 |
305.95 |
62-65 |
S1357-S1765 |
PCR |
yRP6a5, yRP16b1 |
no, prox. to D11S1765 |
n.v. |
- |
|||
|
EST21946c |
T02918 |
305.95 |
62-65 |
S1357-S1765 |
PCR |
dJ59L21 |
yes |
no signal |
- |
|||
|
D11S2319Ec |
Z42911 |
302.7 |
62-70 |
S1357-S913 |
PCR |
dJ71C4, dJ16F23 |
yes |
+ |
TU4 |
|||
|
EST277255 EST274430c |
H17317 H14492 |
303.74 306.35 |
62-65 - |
S1357-S1765 |
PCR |
dJ71C4, dJ16F23 |
yes |
+ |
} |
TU5 |
||
|
EST142190c |
T79827 |
305.92 |
62-65 |
S1357-S1765 |
PCR/H |
dJ71C4, dJ16F23, dJ387P6 |
yes |
+ |
TU7 |
|||
|
D11S2270Ec D11S2224Ec |
Z38928 M85848 |
305.95 - |
62-65 - |
S1357-S1765 - |
PCR |
dJ71C4, dJ387P6 |
yes |
+ |
} |
TU8 |
||
|
EST173048c |
R02752 |
306.05 |
62-65 |
S1357-S1765 |
PCR |
dJ71C4, dJ16F23 |
yes |
+ |
TU6 |
|||
|
EST387646 |
H85442 |
317.19 |
62-65 |
S1357-S1765 |
H |
dJ387P6, dJ455M17 |
yes |
+ |
TU9 |
|||
|
EST501970 |
N95172 |
296.95 |
62-65 |
S1357-S1765 |
H |
- |
no |
n.v. |
- |
|||
| EST118255 |
T63776 |
300.80 |
62-65 |
S1357-S1765 |
PCR |
- |
no |
n.v. |
- |
|||
|
HSU20659d |
U20659 |
304.12 |
62-70 |
S1357-S913 |
PCR |
- |
no |
n.v. |
- |
|||
|
EST287524 |
H26977 |
306.25 |
62-65 |
S1357-S1765 |
PCR |
- |
no |
n.v. |
- |
|||
|
EST389122 |
H86813 |
343.86 |
65-70 |
S1765-S913 |
H |
- |
no |
n.v. |
- |
|||
|
EST394503 |
H92093 |
344.07 |
62-70 |
S1357-S913 |
H |
- |
no |
n.v. |
- |
|||
|
EST117430 |
T62803 |
344.81 |
65-70 |
S1765-S913 |
H |
- |
no |
n.v. |
- |
|||
|
EST241393 |
R64638 |
- |
62-70 |
S1357-S913 |
H |
- |
no |
n.v. |
- |
|||
ESTs mapping to the YAC/PAC contig of the Best's disease region are ordered from centromere to telomere, the order of the remaining ESTs is based on their RH map position.
aESTs are named either by GDB D numbers or by dbEST Ids. bMapping data from the RH mapping consortium can be viewed at http://www.ncbi.nlm.gov/UniGene /index.html. cEST clones have been mapped by Cooper et al. 1997. d Locus HSU20659 defines Human RNA polymerase II subunit hsRPB7.
n.v., not verified; H, hybridization; dJ, denotes PAC clones; yRP, RPCI YACs; 942_g_8, CEPH MegaYAC.
This Article
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doi: 10.1101/gr.8.1.48 Genome Res. January 1, 1998 vol. 8 no. 1 48-56
