Resource

ProPhylER: A curated online resource for protein function and structure based on evolutionary constraint analyses

    • 1Stanford University School of Medicine, Departments of Pathology and Genetics, Stanford, California 94305, USA;
    • 2Wellesley, Massachusetts 02482, USA;
    • 3North Carolina State University, Departments of Statistics and Genetics, Raleigh, North Carolina 27695, USA
Published October 21, 2009. https://doi.org/10.1101/gr.097121.109
Download PDF Cite Article Permissions Share
cover of Genome Research Vol 36 Issue 7
Current Issue:
July 2026, Vol. 36, No. 7
Focus view

Abstract

ProPhylER (Protein Phylogeny and Evolutionary Rates) is a next-generation curated proteome resource that uses comparative sequence analysis to predict constraint and mutation impact for eukaryotic proteins. Its purpose is to inform any research program for which protein function and structure are relevant, by the predictive power of evolutionary constraint analyses. ProPhylER currently has nearly 9000 clusters of related proteins, including more than 200,000 sequences. It serves data via two interfaces. The “ProPhylER Interface” displays predictive analyses in sequence space; the “CrystalPainter” maps evolutionary constraints onto solved protein structures. Here we summarize ProPhylER's data content and analysis pipeline, demonstrate the use of ProPhylER's interfaces, and evaluate ProPhylER's unique regional analysis of evolutionary constraint. The high accuracy of ProPhylER's regional analysis complements the high resolution of its single-site analysis to effectively guide and inform structure–function investigations and predict the impact of polymorphisms.

Back to top
Article contents

Announcement(s)