BAC clone contig and locations of STSs across a 2.2-Mb region of human chromosome 1q24. (Left) 1q telomere; (right) centromere. The Figure is drawn to scale; the length of individual BAC clones (solid rectangles) reflects measured insert sizes. The locations of STSs are listed across the top with shaded lines extending through the contig as guides. Groups of STSs that are not ordered with respect to one another are indicated by a solid line under the STS names. STSs for which location was not strictly constrained were spaced at roughly equidistant intervals between constrained markers. Some STSs contain two names separated by a (/) to indicate that two independent primer sets were tested on the contig and subsequently determined to be from the same location. We have added a prefix C to the addresses of three clones obtained from the CEPH BAC library (C283F8, C207A5, C258G3). STS names that are a four digit number beginning with 2 are BAC end STSs derived from contig clones. (See Table 1 for the correspondence between STS number and clone insert end.) YAC end STSs L733F12, R902C7, L928G11, R912H6, L766E4, L885B7, R821E8, R834F6, and R719G2, are from Belmouden et al. (1997), and 650G9L and 933H12L are from Clépet et al. (1996). D1S3666 is from Sunden et al. (1996). C1E13 and C1E117 are from Seltmann et al. (1994). See Table 2 for STSs from genes and ESTs. Primer pairs for FMO1-4 were obtained from the literature (Dolphin et al. 1991, 1992; Shephard et al. 1993; McCombie et al. 1996). The remaining STSs are from publicly available physical maps from the Whitehead Institute Center for Genome Research, NCBI, or the Stanford Human Genome Center.
