Isolation of Zebrafish gdf7 and Comparative Genetic Mapping of Genes Belonging to the Growth/Differentiation Factor 5, 6, 7 Subgroup of the TGF-β Superfamily

Nucleotide and predicted amino acid sequence of zebrafishgdf7. The ORF contains two potential proteolytic processing motifs (R-X-X-R); site 1 (boxed and shaded) and site 2 (boxed). Three N-linked glycosylation sites are located within the prodomain (underlined and in italics) and the mature domain contains seven conserved cysteine residues (circled). The sequence of the putative 3′ UTR contains two potential polyadenylation signals (thick underlined). (Arrowhead) Boundary between sequence obtained by 5′ RACE and sequence obtained from genomic DNA. The GenBank accession nos. for zebrafish gdf7 are AF113022 and AF113023.