Figure 3.
RT–PCR temporal expression profiles of NR genes on Chromosome III. RT–PCR reactions were performed as described in Materials and Methods. RNA samples were from staged cultures of embryos (E), larval stages L1–L4, and adults (A) or from mixed stage cultures (M). PCR amplification of genomic DNA (G) was included for comparison. Sizes of DNA markers (m) are indicated. Products of the sizes predicted (see Materials and Methods) from amplification of genomic DNA or cDNA samples are designated. For nhr-6, cDNA2 indicates the smaller than expected product obtained from early stages.
