Expression patterns of wrt and grd reporter constructs. Worms are oriented with anterior to the left and ventral downward unless indicated otherwise. (A) Fluorescent micrograph of L1 larva: wrt-4M::gfp is expressed in hypodermal syncytia and the subventral P blastomeres. (B,C) wrt-2M::gfp expression in all seam cells of an L1 larva (B) and an 1.8-fold stage embryo (C). (D) Head of L3 larva. wrt-3::gfp is expressed in pharyngeal gland cell g1; arrows point to the three nuclei of this large cell, arrowheads show two of the processes. (E)wrt-6::gfp expression in socket cells of the amphids (Amso) and one of the inner labial sensilla (Ilso) is shown. hyp5, an anterior hypodermal cell, is indicated. (F) L1 larva, dorsal view.wrt-5::gfp is seen in the seam cells, excretory cell (arrowhead), syncytial hypodermis, the six pharyngeointestinal valve cells (pi), rectal cells (re), and the pharynx corpus. (G)grd-1M::gfp is expressed in the rectal epithelial cells rect D, rect VL, and rectVR. (H) A posterior DA motorneuron expressing grd-7M::gfp; the nucleus (arrow) and axon (arrowheads) are shown. (I–K) grd-6C::gfp–lacZ is expressed in neurons of the head (I) and tail (K) as well as the bilateral HSN neurons (J, ventral view) of adult animals. The axon (arrowhead) belongs to the left nucleus (out of focus, toparrow); the right HSN nucleus is in focus (bottom arrow). The axon's twist is due to use of therol-6 coinjection marker. (L,M)grd-3C::gfp–lacZ (L) and grd-5C::gfp–lacZ(M) are expressed weakly in anterior seam cells of adults (arrows).
