Dispersed Repetitive DNA Has Spread to New Genomes Since Polyploid Formation in Cotton

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Figure 7.
Figure 7.

Hybridization of radioactively labeled first-strand cDNA made from seedling poly(A)+ RNA to 1 μg of slot-blotted DNA from the individual repetitive clones. Hybridization intensity is expressed as a ratio of signal (quantified by densitometry) from hybridization with labeled cDNA to signal from hybridization with labeled plasmid DNA. Triplicate values for the negative control (1 μg of slot-blotted plasmid DNA) averaged 0.09. A total of 12 individual repetitive DNA clones showed ratios of >10, or 100 times the negative control. DNA sequences corresponding to high-signal repetitive DNA clones are indicated.

This Article

  1. Genome Res. 8: 479-492

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