Slot-blot hybridization analysis of genome specificity for cotton dispersed repetitive DNA elements. Slot-blotted genomic DNA from 19Gossypium taxa and an outgroup, hybridized with (A) pXP137, representing an A-genome-specific family; (B) pXP195, representing a D-genome-specific family; (C) pXP224, representing an A-genome-enriched family; (D) pXP215, representing a family common to A and D genomes. Starting with slot 1, genome types and individual taxa are: D genome: (1) G. aridum #123; (2) G. laxum D9-3; (3)G. thurberi D10-9; (4) G. trilobum D8-4; (5) G. klotschianum D3k-55; (6) G. davidsonii #32A; (7) G. raimondii D5-37; (8) G. gossypioides. A genome: (9) G. arboreum, (10) G. herbaceum. AD tetraploid: (11) G. hirsutum Tx9; (12) G. barbadense K101. B genome: (13) G. anomalum;(14) G. triphyllum. C genome: (15) G. robinsonii; (16) G. sturtuanum var. nandewarense AZ40. E genome: (17) G. somalense E2. F genome: (18) G. longicalyx. G genome: (19) G. bickii G1-4. Outgroup: (20) T. lampas. Prior genomic Southern analysis showed that the five tetraploid species are similar in their set of repetitive elements (Zhao et al. 1995); consequently, only two were used here.
