Modification of Bacterial Artificial Chromosome Clones Using Cre Recombinase: Introduction of Selectable Markers for Expression in Eukaryotic Cells

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Figure 4.
Figure 4.

FACS analysis of GFP expression in transfected SW480 cells performed 2 days after transfection of modified or unmodified BAC DNA. (A) Profile of cells transfected with DNA from parental nonretrofitted BAC clone 222N15 shows low-level background fluorescence (0.13%) of gated viable cells. (B) Cells transfected with DNA from the retrofitted BAC clone shows a population emitting green fluorescence (5.73%) using markers from 1.4 to 3.7 log fluorescence.

This Article

  1. Genome Res. 8: 404-412

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