Bacterial Contig Map of the 21q11 Region Associated with Alzheimer’s Disease and Abnormal Myelopoiesis in Down Syndrome

BAC B62L20 and PAC 29H4 are a pair of overlapping clones, and PAC 82L10, a third clone from the MTP crossing parts of this region (see Fig. 1). Two rodent background hybrids containing (as the only human chromosomes) the whole chromosome 21 (WA17) and the whole chromosome 13 (GB3) were used. The cell line A9 was used as the mouse background for WA17, and the CHO as the hamster background control for GB3. All marker primer combinations and PCR conditions were as in GDB. Asterisks indicate that results were obtained by genomic Southern blot hybridization; (FISH:clone name) result obtained using the clone as a probe for FISH on normal human metaphases. The chromosome localization column shows data taken from Graw et al. (1995) ^a and Chiang et al. (1995) ^b, for comparison, which is necessary to deduce the chromosome 21 origin of the three BAC/PAC clones shown. The result for D21S258 was performed in this study, on the whole genome panel of monochromosomal hybrids.