Summary of the Hybridization and PCR-based Hits That Establish the Overlaps within the MTP
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(Left) Sources of probes; (top) target recombinant clones. All clones belong to the MTP (see Fig. 1) except 256M13 and 184J15. The exact type of probe used in each probe target combination was (1) inverse PCR T7 end; (2) inverse PCR SP6 end; (3) vectorette T7 end; (4) vectorette SP6 end; (5) PAC/BAC clone insert; (6) PACAlu PCR products; (7) cosmid insert fragments; (8) T7 riboprobe; (9) marker by PCR; (10) marker by hybridization; (11) gene or EST by PCR; (12) gene or ES by hybridization. ES sequences are described in the text and in Cheng et al. (1994), Yaspo et al. (1995), and Schuler et al. (1996); sequences for gene probes (see Methods) for STCH and RIP 140 are described in Brodsky et al. (1995) and Cavailles et al. (1995), respectively; markers from the GDB start with an S, the prefix D21 is omitted; cosmids start with the prefix c102, BACs start with the letter B, and the rest are PACs.
