Physical and Genetic Mapping of the Human X Chromosome Centromere: Repression of Recombination

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Figure 2.
Figure 2.

Pulsed-field gel analysis of the DXZ1 array in LT690. High molecular weight DNA was digested with one or two restriction enzymes, separated by electrophoresis, and probed with a DXZ1-specific probe. Fragment sizes were used to derive the restriction map shown in Fig 1A. Enzymes used for each digestion are BglI (lane 1);BglI/BglII (lane 2);BglII (lane 3);KpnI/BglII (lane 4); KpnI (lane 5); BglI/KpnI (lane6); BglI (lane 7);BglI/ScaI (lane 8); ScaI (lane 9); ScaI/KpnI (lane 10);KpnI (lane 11); ScaI/BglII (lane 12); BglII (lane 13);ApaI (lane 14); ApaI/BglI (lane 15); ApaI/BglII (lane16); ApaI/KpnI (lane 17);ApaI/ScaI (lane 18). The image is composed of two halves of the same gel, electronically spliced between lanes13 and 14. Selected S. cerevisiae size markers are shown at the right.

This Article

  1. Genome Res. 8: 100-110

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