Figure 10.
Transcription start site. The transcription start site was determined by primer extension as described in Methods. Lanes marked C, T, A, and G contain the bands from the sequencing gel in which the corresponding dideoxynucleotide chain-termination sequencing reactions were electrophoresed alongside the primer extension reaction product, using the same primer. The position of the primer extension band at −447 and the sequence coordinates are indicated to the right.
