Figure 3.
Base-call quality and confidence thresholds. (A) A sequence trace from an AT-rich region of a 1.2-kb HIV target is shown. Ann base represents a no call (1.15 threshold). The data were generated by standard T4 DNA ligation conditions on an 8-mer array. (B) The base calls for a 1041-bp stretch of the HIV target were ranked and sorted by their confidence score. Incorrect base calls are indicated by a diamond symbol arbitrarily assigned a value of one for pictorial purposes. The red line indicates the confidence threshold of 1.15. The incorrect calls cluster at the lowest confidence scores.
