High-Precision Genotyping by Denaturing Capillary Electrophoresis

Table 4.

Electrophoresis Conditions

Condition Capillary Polymer/urea Voltage (V/cm) Temperature (°C) Run time (min)
GSP1 Lt 41 cm 3%/6.6 m 317 30 21
Ld 30 cm
i.d. 75 μm
GSP2 Lt 61 cm 3%/8 m 246 30 41
Ld 50 cm
i.d. 75 μm
POP-4a Lt 41 cm POP-4 366 60 23
Ld 30 cm
i.d. 50 μm
POP-4b Lt 47 cm POP-4 319 60 31
Ld 36 cm
i.d. 50 μm
  • (Lt) Total length of capillary; (Ld) length of capillary from injection to detector window; (i.d.) internal diameter.

  • Run time includes electrophoresis time plus the times required to refill the capillary after each analysis with fresh polymer, sample injection, and rinsing of the capillary/electrode tip.

This Article

  1. Genome Res. 8: 69-80

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