Table 2.
Sizing Accuracy for Allelic Ladders HUMTHO1
| THO1 allele no. | ES | DE | S-GSP | DS/GSP | S-POP-4 | DS/POP-4 |
| 5 | 154 | 151.79 | 152.29 | |||
| 4 | 6.03 | 4.54 | ||||
| 6 | 158 | 157.82 | 156.65 | |||
| 4 | 5.18 | 4.32 | ||||
| 7 | 162 | 163 | 160.97 | |||
| 4 | 4.4 | 4.11 | ||||
| 8 | 166 | 167.4 | 165.08 | |||
| 4 | 4.2 | 4.06 | ||||
| 9 | 170 | 171.6 | 169.14 | |||
| 3 | 3.21 | 3.10 | ||||
| 9.3 | 173 | 174.81 | 172.24 | |||
| 5 | 4.71 | 4.90 | ||||
| 10 | 178 | 179.52 | 177.14 |
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The sizes for each allele were calculated based on the internal size standard, using the local Southern sizing algorithm. (ES) The allele size as calculated by GS software after separation on an ABI 373 (Robertson 1995); (DE) the expected difference in nucleotides between alleles based on the number of repeat units. The calculated sizes determined for runs with GSP1 (S-GSP) and POP-4a(S-POP-4) are listed. Columns DS show the differences in nucleotides between the indicated alleles for either GSP or POP-4.
