(a) Hybridization of fluorescein-labeled IRE-vectorette PCR products derived from RH XZH962 to normal human metaphase chromosomes (46 XY). The probe hybridizes to a single region (5q32–q33) of chromosome 5 (arrows). Some background hybridization to other chromosomes is evident; however, the human content of the parental hybrid is solely chromosome 5. (b) Chromosomal origin of human DNA in RHs with only one detectable human fragment. The boxed numbers represent the RH identification number; the solid vertical lines indicate the extent of hybridization to the chromosome as depicted in the ideogram. Broken lines connect multiple regions of hybridization observed for a single hybrid. Groups I, II, and III represent hybrids that generated one, two, and three observed regions of hybridization, respectively.
