An extreme example of gene mangling in M. leprae: A region of cosmid B2235 homologous to M. tuberculosisTYSY_MYCTU. (Top line) The asterisks indicate positions of identity between the M. tuberculosis TYSY amino acid sequence (TYSY_MYCTU) and the conceptual translation of bases 4387–5673 of M. leprae cosmid B2235 (Translate). The PairWise (Birney and Thompson 1995) nucleotide triplets are displayed under the corresponding M. leprae amino acids. This represents only one possible ythY reconstruction involving 10 frameshifts (^) and 12 stop codons (#) using the results of analysis with Detect43 and PairWise programs. Additional identities covering the VGQG and AIPVQ sequences can be obtained with different hypothetical mutations. The TBLASTN probability for all GenBank nonredundant (NR) protein sequences at 376, 511, 003 amino acid residues is 6.4 e-67.
