Table 1.
YAC Fragmentation Frequencies of pBP108 (109)/ADE, pBP108 (109)/ADE/CEN, and pRFV2neo/CEN
| Vector | YAC | No. of colonies | No. of correct phenotype/no. tested | No. truncated YACs/ no. tested by PFGE |
| A pBP108/ADE | 939_h_7 | 200–300 | 29/30 (97%) | 29/29 (100%) |
| pBP109/ADE | 939_h_7 | 200–300 | 31/37 (84%) | 31/31 (100%) |
| pBP108/ADE | 960_a_5 | 21 | 20/21 (95%) | 20/20 (100%) |
| pBP109/ADE | 960_a_5 | 43 | 21/23 (91%) | 21/21 (100%) |
| B pBP108/ADE/CEN | 766_a_12 | 408 | 47/100 (47%) | 44/44 (100%) |
| pBP109/ADE/CEN | 766_a_12 | 481 | 38/100 (38%) | 32/32 (100%) |
| pBP108/ADE/CEN | 932_e_9 | 831 | 26/100 (26%) | 23/24 (96%) |
| pBP109/ADE/CEN | 932_e_9 | 940 | 22/100 (22%) | 08/09 (89%) |
| C pRFV2neo/CEN | 766_a_12 | 600–700 | 53/149 (36%) | 06/06 (100%) |
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↵Total number of colonies found on different spreads of the same transformation mixture per 5 μg of vector.
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↵Number of correct phenotype: Ade+ Ura−(A); Ade+ Trp− (B); and His+Trp− (C). Number tested: Number of colonies transferred manually or by replica plating to a plate selecting for the targeted YAC arm; upon loss of the original YAC arm, colonies become Ura− (A) or Trp− (B and C).
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↵16%–38% of the colonies were red; these were not analyzed further.
