Centromeric and Noncentromeric ADE2-Selectable Fragmentation Vectors for Yeast Artificial Chromosomes in AB1380

  1. Joris J. Heus1,
  2. Menno P.J. de Winther,
  3. Esther van de Vosse,
  4. Gert-Jan B. van Ommen, and
  5. Johan T. den Dunnen2
  1. Department of Human Genetics, Leiden University, 2333 AL Leiden, The Netherlands

Abstract

We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 orHIS5 as the selectable marker, enabling direct use in AB1380, the host strain of most publicly available YAC libraries. Centromeric fragmentation vectors for AB1380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation.

Footnotes

  • ↵1 Present address: Pharming B.V., Niels Bohrweg 11-13, 2333 CA, Leiden, the Netherlands.

  • ↵2 Corresponding author.

  • E-MAIL ddunnen{at}ruly46.medfac.leidenuniv.nl; FAX +31(0)71-527 60 75.

    • Received December 2, 1996.
    • Accepted April 4, 1997.
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  1. doi: 10.1101/gr.7.6.657 Genome Res. 7: 657-660 Cold Spring Harbor Laboratory Press

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