Figure 2.
YAC fragmentation panel of YAC 939_h_7 made using pBP108/ADE2 and pBP109/ADE2. Fragmentation YACs were ordered by size (deduced from an initial gel) and separated on a PFGE gel, which was blotted and probed with a 270-bp Alu fragment (isolated from pBP108 by BamHI digestion). The conditions used were 0.6% SeaKem Gold agarose, 1× PFG buffer (0.045 mTris, 0.045 m boric acid, 0.2 mm EDTA at pH 8.3), for 44 hr, at 100 V with 20–500 sec pulse time. The extra band indicated by the arrow may be the result of an impure YAC clone.
