Minisequencing: A Specific Tool for DNA Analysis and Diagnostics on Oligonucleotide Arrays

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Figure 2.
Figure 2.

Image obtained from the analysis of eight samples carrying heterozygous mutations by minisequencing on a primer array. The minisequencing primers for detecting nine mutations were immobilized on the arrays in the following order: (Top row, left to right), INCLFin, AGUFin, and FVLeiden; (middle row), FHHki, FHNK,and FHTku; (bottom row) GA1, GA2, and NKH1. The [33P]ddNTP included in the minisequencing reactions is indicated at the top; the genotypes of the samples with the [33P]ddNTP expected to be incorporated at the site of the mutation are given at left.

This Article

  1. Genome Res. 7: 606-614

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