Genomic sequence data encompassing the PEX gene region in Xp22.1. This data has been entered into an ACeDB database, to assimilate better the results from various analysis programs. The scale bar indicates the length of the sequence in base pairs. In the shaded box to the left of the scale bar is the contiguous genomic sequence (one contig of ∼243 kb). Cosmid clones are black bars atleft. In total, the sequence was derived from nine overlapping cosmids. The predicted protein structure of PEX is represented schematically at right. Within this, the uppermost black box represents a transmembrane domain, and the two thinner black boxes represent conserved zinc-binding motifs (HEXXH and ENXADXGG), containing the three zinc-binding ligands (H, H, and E). ThePEX gene consists of 22 exons spanning 220 kb. The genomic organization of PEX is indicated to the left of thePEX protein. The results for the forward strand from three different gene prediction programs are depicted in the shaded box to the right of the scale bar. Comparing the results of these three programs with the known exons from the PEX gene, it can be seen that Grail predicts 18 of the 22 PEX exons (and 27 additional exons), whereas FEXH predicts 19 of the 22 PEXexons (and 35 additional exons). Of the additional exons predicted (non-PEX), only four are predicted in common between the two programs. This genomic sequence contains the markers DXS8254 (at position 8.2 kb) and DXS1683 (at position 228 kb). Nucleotides 1–28835 were found to overlap with a sequence already submitted to GenBank (accession no. U73024).
