Expression Analysis of 12 New Putative Genes on 5q31 Discovered Based on Homology to Previously Characterized Genes
| Putative gene | Homology, function, and related comments | BLASTX (P value) | RT–PCR analysis of cDNA libraries |
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| Number of tissue derivations of EST matches | Size and expression patterns of transcripts by Northern blot analysis | RNase protection assay using YAC transgenic mice |
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↵The percent amino acid identity and NCBI accession numbers of the homologous proteins are indicated in brackets.
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↵Signal strengths are represented as follows: Strong (++); moderate (+); weak (+−); negative (−).
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↵Two ESTs can be developed from the 5′ and 3′ ends of the same cDNA clone. The number of cDNA clones isolated from each tissue is indicated in the first bracket. The NCBI accession number of only one EST derived from each tissue is given in the second bracket.
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Tenative human consensus sequences (THCs) are composed of two or more cDNA clones that have been assembled based on overlap into a larger block of sequence than a typical EST (Adams et al. 1995).
