An STS-Based Radiation Hybrid Map of the Human Genome
- Elizabeth A. Stewart,
- Kathleen B. McKusick,
- Amita Aggarwal,
- Ewa Bajorek,
- Shannon Brady,
- Angela Chu,
- Nicole Fang,
- David Hadley,
- Mark Harris,
- Sami Hussain1,
- Richard Lee,
- Annu Maratukulam,
- Kyle O’Connor2,
- Shanti Perkins,
- Mark Piercy,
- Fawn Qin,
- Tim Reif,
- Carla Sanders,
- Xiaohong She,
- Wei-Lin Sun,
- Poroshat Tabar,
- Susan Voyticky,
- Sid Cowles3,
- Jian-Bing Fan1,
- Chris Mader,
- John Quackenbush,
- Richard M. Myers, and
- David R. Cox4
Abstract
We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is <5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb.
Footnotes
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Present address: 1Affymetrix, Inc., Santa Clara, California 95051; 2Millenium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139-4815; 3Incyte Pharmaceuticals, Inc., Palo Alto, California 94394.
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↵4 Corresponding author.
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E-MAIL cox{at}shgc.stanford.edu; FAX (415) 725-8058.
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- Received November 13, 1996.
- Accepted March 7, 1997.
- Cold Spring Harbor Laboratory Press
