A typical agarose gel showing the results of PCR testing of transgenic strains. The 380-bp band in all experimental lanes is the product of the control primers R5 and R11, which were included in all reactions. (Lane A) Results of performing PCR on DNA obtained from an N2 worm that has been cotransformed with a pJB8 vector cosmid and pCes1943. Primers used were DC1 and DC2. (Lane B) Results obtained from an N2 worm that has been transformed with pCes1943 only. Primers used were DC1 and DC2. (Lane C) Results obtained from an N2 worm that has been cotransformed with a Lorist vector cosmid and pCes1943. Primers used are Krp17 and Krp18. (Lane D) Results obtained from an N2 worm that had been transformed with pCes1943 only. Primers used are Krp17 and Krp18. (Lane M) DNA size markers (Promega 1 kb ladder).
