Anemia- and BMP4-sensitive chromatin accessibility changes associated with common human SNPs. (A) Experimental layout. (B) Dot plot representing scaled single-cell mRNA expression per cell (color) and fraction of cells expressing (size) within each annotated cell population isolated from mice 3 days post-PHZ versus control cells. Gene list was filtered to those located at BMP4 and anemia-sensitive genomic regions, containing SNPs which are sensitive to BMP4 stimulation. (C) Volcano plot depicts all anemia-regulated genes in HSPCs and erythroid cells, annotated by loci containing human polymorphisms that may alter expression profiles and SNPs that were significantly associated with differential sensitivity to BMP4 stimulation. (D) TOBIAS Footprint scores at the Cpd locus at days 1, 3, and 7 post-PHZ mapped to the mouse mm10 genome. (E) Inset represents the corresponding region in the human genome (GRCh38) depicting the location of the BMP4-sensitive SNP within the CPD promoter, JUN ChIP in K562 (GEO: GSM935355), and layered H3K27ac from ENCODE (The ENCODE Project Consortium 2012).
