Broad chromatin accessibility changes at intermediate stages of anemia resolution. (A) Experimental layout. (B) Principal component analysis of ATAC-seq peak data comparing control spleen to post-PHZ time points at days 1, 3, 7, 14, 21, and 35 (N = 3). (C) Heat map of Z-score changes in ATAC-seq peak signal among a cohort of sites identified as differentially accessible using DESeq2 (likelihood ratio test) and k-means clustered. (D) Line graphs of ATAC-seq peak accessibility within each state (Z-score). (E) Ranked variability scores of individual transcription factor (TF) motifs in anemia-regulated ATAC-seq peaks. Inset depicts a heat map representation of ChromVAR Z-scores at the top 20 motifs identified by variability. (F) Volcano plot of TOBIAS differential binding footprint scores between days 7 and 14 post-PHZ.
