Anemia-induced changes to AP-1 expression correspond with accessibility at erythropoiesis-promoting gene loci. (A) Volcano plot in single-cell RNA-seq data from PHZ-treated mice depicts expression changes in erythroid cells after 24 h, TF = transcription factor. (B) Dot plot depicting single-cell RNA-seq analysis of HSPC and erythroid-specific expression of all detectable components of the AP-1 transcription factor family at 0, 1, 3, and 7 days post-PHZ. (C) Heat map of genomic regions with changes to TOBIAS footprint scores between 0 and 1 day post-PHZ, ranked by fold change. (D) SCENIC analysis comparing the regulon activity of AP-1 components in HPSCs at 0, 1, 3, and 7 days post-PHZ. (E) Kif3b and Osm mRNA expression plots from single-cell RNA-seq data in cells annotated as HSPCs and erythroid clusters. Units of expression are relative log normalized. Error bars represent SD. (***) P < 0.001 (Wilcoxon signed-rank test).
