Figure 4.
Simultaneous targeting of seven genes using V2.1G. (A) Schematic for targeting seven endogenous genes in PFFs using V2.1G. A surrogate reporter (eGFP) plasmid is used for selecting B4GALNT2-targeting cells. After 24 h of transfection, cells were sorted into three groups: V2.1G (FITC-highest), Enrich (PE-highest), and Control (PE-positive), for genotyping and colony formation. (B) Genotyping of the clones for seven genes at 16 alleles. Each box represents one allele, filled boxes represent a targeted allele, and empty boxes represent a nontargeted allele.
