Table 2.
Results on ONT data
| Data set | Assembler | Size (Mb) | LG50 | LG90 | NG50 (Mb) | NGA50 (Mb) | Complete (%) | Duplicated (%) | QV | # misasm structural | # misasm local |
|---|---|---|---|---|---|---|---|---|---|---|---|
| CHM13 | GNNome | 2984 | 11 | 27 | 111.0 | 83.1 | 99.42 | 0.83 | 33.59 | 1758 | 1695 |
| Raven | 2907 | 16 | 48 | 72.6 | 70.3 | 99.51 | 0.70 | 34.04 | 1146 | 952 | |
| Flye | 2851 | 17 | 60 | 70.0 | 69.5 | 99.50 | 0.69 | 33.91 | 134 | 123 | |
| Shasta | 2884 | 17 | 72 | 59.7 | 56.3 | 99.50 | 0.55 | 37.30 | 57 | 190 | |
| A. thaliana | GNNome | 131 | 4 | 9 | 14.6 | 14.5 | 99.83 | 1.07 | 26.54 | 242 | 221 |
| Raven | 125 | 5 | 11 | 14.5 | 14.5 | 99.81 | 1.07 | 27.28 | 95 | 185 | |
| Flye | 124 | 5 | 15 | 13.7 | 13.7 | 99.79 | 1.07 | 26.03 | 7 | 16 | |
| Shasta | 115 | 5 | — | 12.1 | 12.1 | 99.81 | 0.94 | 25.84 | 16 | 256 |
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The best-achieved results are in bold. The assemblies of GNNome and Raven were additionally polished with Racon (Vaser et al. 2017). Flye and Shasta have built-in polishing modules, so we did not perform additional postassembly polishing. Shasta did not assemble 90% of the A. thaliana genome, hence the LG90 is undefined. Full QUAST report for ONT data is given in Supplemental Table S2.
