Post-transcriptional cross- and auto-regulation buffer expression of the human RNA helicases DDX3X and DDX3Y

DDX3X is negatively auto-regulated in 46,XX cells. (A) DDX3X’s allelic ratio (AR) is significantly higher than its ΔE_X value in LCLs, setting it apart from all other Xi/Xa/Y-expressed X–Y pair genes, whose AR values approximate their ΔE_X values. Statistical significance determined via one sample t-test: P = 0.02. (B) DDX3X transcript levels (by qPCR) in 46,XX fibroblasts are significantly elevated in a dose-responsive manner upon treatment with DDX3 helicase inhibitor RK-33. Statistical significance determined by one-sided t-test on delta Ct values. Error bars indicate the standard deviation of three technical replicates. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001.