Specialized cell types and developmental stages are distinguished within major tissue type divisions. (A) UMAP projections showing normalized expression profiles of select DEGs within the atlas showing cluster specificity. Names of Arabidopsis orthologs are given (Supplemental Data Set S10). (B) UMAP showing that expression of Wa8730a007g001710, ortholog of Arabidopsis FAMA, is concentrated in a small subset of cells within supercluster CD. (C) Stomata, marked by autofluorescence (green), are dispersed across the upper, above-water, epidermis. White arrows highlight select guard cells. Scale bar, 250 µm. (D) UMAP visualization of “gene set activity” (Seurat) of daughter-enriched DEGs determined by bulk RNA-seq shows that developmentally active daughter cells are localized primarily within specific clusters, particularly within a region of C6. (E) UMAP visualizations of “gene set activity” of genes associated with distinct phases of the cell cycle (Supplemental Data Set S11) show that although cells undergoing DNA replication are largely dispersed across the atlas, mitotic cells primarily localize to C6 and, to a lesser extent, C4, mirroring the distribution of daughter cells.
