H3K9me3 and H3K27me3 regulate the expression levels of genes known to be related to diapause regulation. (A) Relative expression changes of genes in the juvenile hormone (JH) pathway after treatment with 5 mg/kg of the SUV39H2-like inhibitor chaetocin and 10 mg/kg of the E(Z) inhibitor tazemetostat compared with DMSO controls. Statistical differences were measured using an unpaired t-test: (ns) no significant difference, (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, and (****) P < 0.0001. Error bars show the mean ± SEM. (B) Percentage of diapause was significantly changed by treatment of 0.01 mg/kg JH with or without 5 mg/kg of the SUV39H2-like inhibitor chaetocin or 10 mg/kg of the E(Z) inhibitor tazemetostat, respectively. Statistical differences were measured using an unpaired t-test: (ns) no significant difference, (****) P < 0.0001. Each data point represents the value of an independent replicate.
