Analysis on the trend of global-level changes in three modifications and their correlations with gene expression levels during diapause. The three epigenetic modifications show significant changes during the diapause stages of Asian corn borer larvae. (A) Heatmaps illustrating the dynamics of H3K9me3 (left), H3K27me3 (middle), and gene counts (right) of all genes bearing the H3K9me3 domain in the heads of Asian corn borer larvae. (B–D) Box-plots indicating the average levels of H3K9me3, H3K27me3, and gene counts of all gene-locus-bearing H3K9me3 domains at the nondiapause, prediapause, and diapause stages. Each box represents the mean ± SEM. (****) P < 0.0001 (determined by unpaired t-test). (E) Heatmaps showing the dynamics of H3K27me3 (left), H3K9me3 (middle), and gene counts (right) of all genes bearing the H3K27me3 domain in the heads of Asian corn borer larvae. (F–H) Box-plots indicating the average levels of H3K9me3, H3K27me3, and gene counts of all gene-locus-bearing H3K27me3 domains at the nondiapause, prediapause, and diapause stages. Each box represents the mean ± SEM. (****) P < 0.0001 (determined by unpaired t-test). (I) Heatmaps showing the dynamics of DNA methylation (left) and gene counts (right) of all genes bearing DNA methylation domains in the heads of Asian corn borer larvae. (J,K) Box-plots showing the average level of DNA methylation and gene counts of all gene-locus-bearing DNA methylation domains at the nondiapause, prediapause, and diapause stages. Each box represents the mean ± SEM. (****) P < 0.0001 (determined by unpaired t-test). (L–N) Genome-wide correlation plots of DNA methylation and gene counts in the nondiapause, prediapause, and diapause stages.
