H3K9me3 and H3K27me3 are involved in diapause regulation of O. furnacalis larvae. (A–E) Western blot analysis was conducted on the head of Asian corn borer larvae to detect the levels of H3K9me3, H3K27me3, H3K4me2, H3K14ac, and H3K27ac. The antibody against total H3 was used as the loading control. The relative gray values of western blot were measured using Image J. Statistical differences were measured using an unpaired t-test: (ns) no significant difference, (**) P < 0.01. Error bars show the mean ± SEM. (F) Newly molted fourth instar larvae were fed artificial diets containing 0 mg/kg and 5 mg/kg SUV39H2-like (homologous to SU(VAR3-9) of D. melanogaster) inhibitor chaetocin, respectively. The results showed that chaetocin alleviated diapause caused by adverse environment. Statistical analysis using an unpaired t-test revealed a significant difference: (**) P < 0.01. (G) Similarly, newly molted fourth instar larvae were fed artificial diets containing 0 mg/kg and 10 mg/kg E(Z) inhibitor tazemetostat, which is homologous to E(Z) of D. melanogaster. The results indicated that tazemetostat exacerbated diapause caused by adverse environmental conditions. Statistical analysis using an unpaired t-test revealed a significant difference: (**) P < 0.01. Each point on the bar graph represents the value of an independent replicate.
