Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy

Dgcr8 KO ESCs show an aberrant transcriptome. (A) PCA plot of wild-type and Dgcr8 KO transcriptomes in normal and MTORi (day 2) conditions. (B) Volcano plots showing differentially expressed genes (Padj < 0.05, log₂FC > 1) in wild-type and Dgcr8 KO cells in normal and MTORi conditions. Dgcr8 KO shows more DE genes (738 vs. 403 in wild-type, 153 in common) and an overall higher significance level. (C) Scatter plot showing fold change (MTORi/normal) of each gene in wild-type and Dgcr8 KO cells. Dgcr8 KO cells show a wider log₂FC range. DE genes in each background and common DE genes are highlighted with colors. (D) Gene Ontology analyses of significantly down-regulated or up-regulated genes in wild-type and Dgcr8 KO cells in normal and MTORi conditions. Commonly regulated pathways in both backgrounds are shown together with those that differ based on the genetic background. Top 10 terms are shown; the full list is provided in Supplemental Table S2.