Fertility and differential mRNA expression in rde-1 mutants. (A) Each data point is the number of progeny produced by a single wild-type or rde-1Δ(ram40) mutant animal grown at 25°C. The orange bars show the means and the black error bars show the 95% confidence intervals. A two-sample t-test was used to calculate the P-value. n = 8 animals per genotype. (B) The scatter plot displays individual transcripts as a function of the average log2-geometric mean-normalized reads from wild-type (x-axis) and rde-1Δ (ram40) mutant (y-axis) mRNA-seq libraries colored by classification or P-value. None of the 23H-RNA loci had P-values < 0.05. n = 3 biological replicates for each strain. Libraries from gravid adults.
