Figure 5.
Competition between Argonautes for 23H-RNAs. (A,B) RPM-normalized reads corresponding to 23H-RNAs, miRNAs, and piRNAs from GFP::ALG-1 (A) or GFP::ALG-2 (B) co-IP and corresponding cell lysate sRNA-seq libraries. Two-sample t-tests were used to calculate the P-values. Bonferroni corrections were applied to account for multiple comparisons. Libraries from gravid adults treated with control (L4440) or rde-1 RNAi. Error bars are SD between two biological replicates. (C,D) RPM-normalized rde-1-matching reads from GFP::ALG-1 (C) or GFP::ALG-2 (D) co-IP and corresponding cell lysate sRNA-seq libraries.
