23H-RNAs have a high affinity for RDE-1. (A) Percentages of RPM-normalized reads in GFP::RDE-1 co-IP and cell lysate sRNA-seq libraries corresponding to each class of small RNAs. n = 2 biological replicates. Libraries are the same as in Figure 2A. (B) Scatter plots display individual small RNA features as the average log2-geometric mean-normalized reads in cell lysates (x-axis) and GFP::RDE-1 co-IPs (y-axis) colored by small RNA class. Exogenous siRNAs matching nrfl-1 and oma-1 are circled. (C) RPM-normalized 23H-RNA reads in wild-type and rde-1(ne219) mutant sRNA-seq libraries. Libraries from gravid adults. Error bars are SD between three biological replicates. The P-value was calculated using a two-sample t-test. (D) Relative levels of the most abundant F43E2.6 siRNA in wild-type and rde-1Δ(ram40) mutants as determined by qRT-PCR and normalized to miR-1 levels. RNA from gravid adults. Error bars are SD between three biological replicates. The P-value was calculated using a two-sample t-test.
