Host and nested genes with high coexpression in the testis can exhibit dynamic coexpression patterns during spermatogenesis at the single-cell level. (A) Scatter plots showing host and nested genes normalized expression level in testis single cells for two example pairs: LINC02253/AC020704.1 and CASC16/AC026462.3. (B) The proportion of cells which coexpress host and nested gene pairs across spermatogenesis. Three distinct coexpression profiles were determined by k-means clustering. (C) Line plot showing the profiles of expression calculated using the method detailed in Supplemental Figure S7C for three different pairs of host and nested genes. GAGE12F/GAGE12E is an example of a pair where host and nested genes are mainly coexpressed; AC073188.2/AC073188.5 is an example of a pair with mutually exclusive expression; and AC1349980.2/OR4M2 is a pair with a complex expression profile in testis.
