Figure 1.
Experimental overview. Full-length cDNA was created from total RNA extracted from 12 BALB/c mouse tissues. Pooled cDNA, both non-size-selected and size-selected (see text), was prepared for ONT sequencing by the R2C2 method. ONT raw reads were demultiplexed and consensus called using C3POa which identifies and combines low-accuracy subreads to create high-accuracy consensus reads. To generate a tissue-level transcriptome for each tissue, R2C2 consensus reads were then processed into isoforms using the Mandalorion pipeline. (SC) spinal cord, (St) stomach, (SM) skeletal muscle, (SG) salivary gland.
