Figure 4.
Nanopore direct RNA sequencing of optimized WAS vectors. Plots showing the sequencing coverage (green), location of the 3′ ends of reads (orange), and splicing patterns (purple lines connecting splice donors and acceptors) for two WAS vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A-to-T mutation in the potential cryptic poly(A) site). (B) WAS LV6 sequencing data (contains an additional two-point mutations in the promoter to remove splice donor sites). (C) Reference sequence of the WAS vector, with various features annotated.
