Figure 3.
Artificially polyadenylated ONT direct RNA sequencing data for a WAS vector. Plots showing the sequencing coverage (green), location of the 3′ ends of reads (orange), and splicing patterns (purple lines connecting splice donors and acceptors) for WAS LV1 vectors sequenced by ONT direct RNA technology, with and without artificial polyadenylation (performed according to Crabtree et al. [2019]). (A) Standard library preparation protocol (no artificial polyadenylation). (B) Artificially polyadenylated sequencing data. (C) Reference sequence of the WAS vector, with various features annotated.
